A serial multiplex immunogold labeling method for identifying peptidergic neurons in connectomes
نویسندگان
چکیده
منابع مشابه
A Serial Multiplex Immunogold Labeling Method for Identifying Peptidergic Neurons 1 in Connectomes 2 3
13 14 Electron microscopy-based connectomics aims to comprehensively map synaptic connections in 15 neural tissue. However, current approaches are limited in their capacity to directly assign molecular 16 identities to neurons. Here, we use serial multiplex immunogold labeling (siGOLD) and serial-17 section transmission electron microscopy (ssTEM) to identify multiple peptidergic neurons in a 1...
متن کاملA serial multiplex immunogold labeling method for identifying peptidergic neurons in connectomes
Electron microscopy-based connectomics aims to comprehensively map synaptic connections in neural tissue. However, current approaches are limited in their capacity to directly assign molecular identities to neurons. Here, we use serial multiplex immunogold labeling (siGOLD) and serial-section transmission electron microscopy (ssTEM) to identify multiple peptidergic neurons in a connectome. The ...
متن کاملa framework for identifying and prioritizing factors affecting customers’ online shopping behavior in iran
the purpose of this study is identifying effective factors which make customers shop online in iran and investigating the importance of discovered factors in online customers’ decision. in the identifying phase, to discover the factors affecting online shopping behavior of customers in iran, the derived reference model summarizing antecedents of online shopping proposed by change et al. was us...
15 صفحه اولDeveloping a Method for Identifying Emergencies in Process Industries
Introduction: One of the essential and critical elements for efficient and effective management of emergencies is anticipation and identification of possible types of emergencies. As such, a framework for anticipating and identifying emergencies was designed and tested in two process industries in the form of a case study. Material and Methods: At first, methods for identifying emergency prepa...
متن کاملA reliable method for simultaneous demonstration of two antigens using a novel combination of immunogold-silver staining and immunoenzymatic labeling.
We have developed a reliable and sensitive immunohistochemical staining technique which allows the simultaneous demonstration of two different antigens expressed in or on the same cell (referred to as mixed labeling), together with the evaluation of the general histopathological appearance of the tissue. The staining procedure combines a three-step (streptavidin-biotin) immunogold-silver staini...
متن کاملذخیره در منابع من
با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید
ژورنال
عنوان ژورنال: eLife
سال: 2015
ISSN: 2050-084X
DOI: 10.7554/elife.11147